Composition characterization and biological activity study of Achillea vermicularis extract and extract-loaded nanoparticles

DOĞAN M., KOÇYİĞİT Ü. M., Çetin F. N., YILMAZ SOYDAN N. T., TAŞKIN T.

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, cilt.70, sa.3, ss.1270-1278, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 70 Sayı: 3
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1002/bab.2437
  • Dergi Adı: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Applied Science & Technology Source, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Compendex, Computer & Applied Sciences, EMBASE, Environment Index, Food Science & Technology Abstracts, INSPEC, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1270-1278
  • Anahtar Kelimeler: cytotoxic activity, encapsulation efficiency, enzyme inhibition, CHITOSAN NANOPARTICLES, CARBONIC-ANHYDRASE, ANTIOXIDANT, DELIVERY, PLANT, OIL
  • Marmara Üniversitesi Adresli: Evet

Özet

Antiproliferative activity of Achillea vermicularis extracts was calculated on glial (C6) and keratinocyte (HaCaT) cell lines using XTT assay. It was observed that all extracts of A. vermicularis at the determined concentration were not cytotoxic in HaCaT cell lines. The nanoparticles (NPs) of the extract with the best cytotoxic activity was prepared, and necessary characterization studies were performed. Results showed that NP containing the extract has a lower IC50 value and more cytotoxic activity in C6 cells compared to the only extract. Furthermore, the antiepileptic potentials of these substances were explored in this study. The effect of A. vermicularis extracts on the enzyme activities of carbonic anhydrase I and II isoenzymes (hCA I and hCA II) was measured using spectrophotometry to achieve this goal. A. vermicularis extracts demonstrated high inhibitory activities compared to standard inhibitor (acetazolamide, AAZ), with IC50 values in the range of 5.04-10.8 mu g/ml for hCA I, and 5.40-9.22 mu g/ml for hCA II. High-performance liquid chromatography diode array detector (HPLC-DAD) was used in this investigation to assess the main chemicals found in the extract and NPs. The results showed that the ethanol extract (157.636 mu g/mg extract) and NPs (4.631 mu g/mg extract) had a significant amount of the 8-hydroxy salvigenin component.