Production and characterization of a high molecular weight levan and fructooligosaccharides from a rhizospheric isolate of Bacillus aryabhattai

Nasir A., Sattar F., Ashfaq I., Lindemann S. R., Chen M., Van den Ende W., ...Daha Fazla

LWT-FOOD SCIENCE AND TECHNOLOGY, cilt.123, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 123
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.lwt.2020.109093
  • Dergi Adı: LWT-FOOD SCIENCE AND TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Compendex, Food Science & Technology Abstracts, Veterinary Science Database
  • Anahtar Kelimeler: Bacillus aryabhattai, Levan, Fructooligosaccharides, Exopolysaccharide, Prebiotics, STRUCTURAL-CHARACTERIZATION, FRUCTANSUCRASE ENZYMES, LEVANSUCRASE, OPTIMIZATION, STRAIN
  • Marmara Üniversitesi Adresli: Evet

Özet

Bacillus aryabhattai GYC2-3 strain, capable of synthesizing EPS, was isolated from the rhizosphere of Taraxacum spp. plant. The EPS was identified as levan by C-13 NMR spectroscopy and methylation analysis. The levan produced had a high weight average molecular weight of 5.317 x 10(7) Da and degree of branching 5.19%. Thin layer chromatography and high-performance anion-exchange chromatography revealed that it is capable of producing broad range of fructooligosaccharides (FOS) and higher levels of FOS synthesis were observed with increasing sucrose concentrations. The optimum temperature, initial pH and sucrose concentration for levan biosynthesis were studied to be 30 degrees C, 8.0 and 250 g/L, respectively. Levan production was increased by similar to 38% (26 g/L) when provided aeration as compare to static cultures. To the best of our knowledge, this is the first report on biosynthesis of levan and FOS by a B. aryabhattai strain.