Akademik Veri Yönetim Sistemi
JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS AND CHEMISTS, cilt.106, sa.4, ss.165-170, 2022 (SCI-Expanded)
Extremely halophilic archaeal populations producing protease and lipase enzymes in preservation salts can contaminate hides and skins during the salt-pack curing or brine curing process. The enzymatic activities of these micro-organisms may cause loose grain, resulting in a significant reduction of leather's commercial value. Controlling the growth of these micro-organisms is essential for quality leather manufacture. Therefore, we carried out investigations to inhibit protease and lipase positive extremely halophilic archaeal strains using an antimicrobial agent containing sodium dimethyldithiocarbamate. In this study, the agar dilution method was used to detect the minimum inhibitory concentrations of the antimicrobial agent against proteolytic and lipolytic Halobacterium salinarum, Haloarcula salaria, and Haloarcula tradensis which were isolated from salted sheepskins with red heat. Eighteen concentrations of sodium dimethyldithiocarbamate ranging from 4000 mu g/ml to 0.028 mu g/ml were separately applied to the test isolates. The minimum inhibitory concentration of sodium dimethyldithiocarbamate against Halobacterium salinarum, Haloarcula salaria, and Haloarcula tradensis was 62.5 mu g/ml. In conclusion, the antimicrobial agent containing sodium dimethyldithiocarbamate was highly effective against proteolytic and lipolytic extremely halophilic archaea. Hence, we suggest using this antimicrobial agent in the brine curing of hides and skins to prevent deterioration and increase the commercial value of leather.