Laminar Optical Tomography Applied to Reporter Genes Imaging in Engineered Tissue Constructs

ÖZTÜRK M. S., Lee V. K., Zhao L., Dai G., Intes X.

39th Annual Northeast Bioengineering Conference (NEBEC), Syracuse, İtalya, 5 - 07 Nisan 2013, ss.129-130 identifier identifier

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Doi Numarası: 10.1109/nebec.2013.106
  • Basıldığı Şehir: Syracuse
  • Basıldığı Ülke: İtalya
  • Sayfa Sayıları: ss.129-130
  • Anahtar Kelimeler: fluorescence, reporter genes, tissue engineering, mesoscopic, tomographic, 3D reconstruction, FLUORESCENCE MOLECULAR TOMOGRAPHY, MICROSCOPY
  • Marmara Üniversitesi Adresli: Hayır

Özet

Fluorescence imaging is the main method in assessing tissue engineered constructs. However, with the drive to develop thick engineered constructs, it is imperative to develop new fluorescence based techniques that can image beyond the depth capabilities of current microscopic techniques. Here we report the use of Laminar Optical Tomography (LOT) to image in 3D vascular channels, formed by GFP and mCherry carrier cells, in a thick printed constructs enclosed in a perfusion chamber. Results show the potential of LOT for locating and monitoring transfected cells in thick tissue with spatial accuracy.