The main purpose of this study was to investigate the bacteriological quality parameters of drinking fountains found in the Anatolian side of Istanbul on an annual basis (2014 year/12 month) and their evaluation in terms of human health. Samples were taken monthly from 10 different public drinking fountains for bacteriological analysis for a year in 2014. Bacteriological analysis were carried on using the membrane filtration technique; Coliform bacteria, Escherichia coli, Fecal coliform and Enterococcus counting. 95 samples were collected in total and bacteriological analysis performed on these showed that 46 % of the samples were drinkable and 49 % of them were not drinkable according to the national and international standards for drinking water quality. Additionally, 47,4 % of the samples had Coliform bacteria, 27,4 % had Enterococcus, 17,9 % had Escherichia coli and 14,7 % had Fecal coliform which showed that they were out of the standards. On the other hand, 48,4 % of the samples were compatible with the values indicated in the standards.