Investigating local and long-range neuronal network dynamics by simultaneous optogenetics, reverse microdialysis and silicon probe recordings in vivo

Taylor H., Schmiedt J. T., Carcak N., ONAT F., Di Giovanni G., Lambert R. C., ...More

JOURNAL OF NEUROSCIENCE METHODS, vol.235, pp.83-91, 2014 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 235
  • Publication Date: 2014
  • Doi Number: 10.1016/j.jneumeth.2014.06.031
  • Journal Name: JOURNAL OF NEUROSCIENCE METHODS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.83-91
  • Keywords: T-type Ca2+ channels, HCN channels, Metabotropic glutamate receptors, EEG, Slow waves, Sleep spindles, Delta waves, Thalamus, Cortex, THALAMOCORTICAL NEURONS, CALCIUM-CHANNEL, SLEEP, VITRO, OSCILLATION, CELLS, PACEMAKER, THALAMUS, NUCLEUS, WAVES
  • Marmara University Affiliated: Yes

Abstract

Background: The advent of optogenetics has given neuroscientists the opportunity to excite or inhibit neuronal population activity with high temporal resolution and cellular selectivity. Thus, when combined with recordings of neuronal ensemble activity in freely moving animals optogenetics can provide an unprecedented snapshot of the contribution of neuronal assemblies to (patho)physiological conditions in vivo. Still, the combination of optogenetic and silicone probe (or tetrode) recordings does not allow investigation of the role played by voltage- and transmitter-gated channels of the opsin-transfected neurons and/or other adjacent neurons in controlling neuronal activity.