Demonstration of The Development of Tight-Junctions Between Sertoli Cells In Post-Natal Rat Testis In Days 4-12: Immunohistochemistry and Ultrastructural Observation


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KOLBAŞI B., ÇETİNEL Ş. , KERVANCIOĞLU G.

18th International Microscopy Congress, PRAGUE, Czech Republic, 7 - 12 September 2014

  • Publication Type: Conference Paper / Summary Text
  • Doi Number: 10.1017/s1551929515000073
  • City: PRAGUE
  • Country: Czech Republic

Abstract

LS-13-P-5940 Demonstration of The Development of Tight-Junctions Between Sertoli Cells In Post-Natal Rat Testis In Days 4-12: Immunohistochemistry and Ultrastructural Observation

Kolbasi B.1 , Kervancıoğlu G.2 , Çetinel Ş.1

1Marmara University, School of Medicine, Dept. of Histology and Embryology, İstanbul, TURKEY,

2Kanuni Sultan Süleyman Training and Research Hospital, Infertility Center, Andrology Lab.İstanbul, TURKEY

Email of the presenting author: bircankolbasi@yahoo.com

Introduction: Testicular development plays an important role in male fertility. Gonadal development begins on the 10th day fetal rat. In the 18-19 day the seminiferous tubules are filled with primordial gonocytes in the fetal testis. From postnatal day 10 primordial germ cells divide and differentiate into spermatogonia. Over the next 40days and so, testicular development demonstrates a series of successful developing germ cell layers. For a proper and continous development, the permutation and function of junctions among Sertoli cells play a crucial role. While spermatogonia transfer into spermatid through the blood-testis barrier, they also move from basal compartment to adluminal compartment.

Aim: We aimed to show the consecutive stages of tight junction morphology in the days 4-12 in the neonatal rat testis.

Materials and Methods: In this study rat testis of days 4-12 were obtained and proceeded for both light and electron microscopy. We accepted the date of birth 0. We observed the blood-testis barrier development day by day in neonatal rats by using ZO-1 immunohistochemistry and for ultrastructural examiation tissues were proceeded by ruthenium red in order to visualize the tight-junctions.

Results: In the days 4-7 the interval between Sertoli cells were wider and from the day 8 to 12 the interval became tighter and the 12th day showed the final morphology of tight junction, ZO-1 immunohistochemistry demonstrated the same morphology in accordance with electron microscopy. Conclusion: Ruthenium red observations in electron microscopy demonstrated that tight-junction formation in neonatal rat begins by days 8-12.