Determination of cisplatin, transplatin and amminetri-chloroplatinate by high performance liquid chromatography in one run using 4-methyl-2-thiouracil as derivatizing agent

Arioz F., Yalcin G., Dolen E.

CHROMATOGRAPHIA, vol.49, pp.562-566, 1999 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 49
  • Publication Date: 1999
  • Doi Number: 10.1007/bf02467760
  • Journal Name: CHROMATOGRAPHIA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.562-566
  • Keywords: column liquid chromatography, cis-diamminedichloroplatinum (II), transdiamminedichloroplatinum (II), amminetri-chloroplatinate, 4-methyl-2-thiouracil, PLATINUM(II) COMPLEXES, POSTCOLUMN DERIVATIZATION, PLASMA ULTRAFILTRATE, DIETHYLDITHIOCARBAMATE, URINE, ION, CIS-DICHLORODIAMMINEPLATINUM(II), SPECTROSCOPY, SULFUR
  • Marmara University Affiliated: Yes

Abstract

We have developed a simple, rapid, selective and sensitive method for detecting the antitumour agent cis-diamminedichloroplatinum (II) (cisplatin) (CDDP) and its toxic impurities trans-diamminedichloroplatinum (II) (transplatin) (TDDP) amminetri-chloroplatinat (ATCP) anion using HPLC in one run. By using 4-methyl-2-thiouracil (MTU) as a derivatizing agent, new compounds have been formed from the Pt compounds and separated on a mu-Bondapak C-18 column with isocratic elution and detection at 315 nm.