Akademik Veri Yönetim Sistemi
Applications of Chemistry in Nanosciences and Biomaterials Engineering NanoBioMat 2024 – Summer Edition, Bucuresti, Romanya, 19 - 21 Haziran 2024, ss.140-141
Epilepsy has been prevalent as a neurological disorder since 4000 BC and continues to be one of the most common conditions in modern times. According to 2019 data from the World Health Organization (WHO), epilepsy affects approximately 0.7% of the population. Furthermore, around 5 million individuals receive a diagnosis each year. Antiepileptic drugs are effective in managing seizures and achieving successful treatment for epilepsy. Approximately 20-30% of individuals demonstrate pharmacoresistance, and only a minority of these patients will experience favorable outcomes with surgical surgery. Although antiepileptic medications are effective in managing epilepsy crises for 70% of patients, approximately 20-30% of patients exhibit drug resistance. Hence, the development of an effective treatment for the disease remains challenging. To accomplish this objective, antiepileptic drug loaded microneedle (MN) based drug delivery systems can be alternative treatment for epilepsy.
The manner and rate of drug release from polymer matrices are determined by the selection of MN material and specific construction method. Hydrogels have been utilized in the production of MNs due to their excellent biocompatibility and biodegradability. Gelatin methacryloyl (GelMA) is a hydrogel that is made by modifying gelatin with photocrosslinkable methacrylamide groups. It is both biodegradable and biocompatible, and it can be used to deliver cells and biomolecules to specific areas. DLP printers are a useful instrument for producing hydrogels through photo-curing, thanks to their rapid printing speed and In this study, drug loaded bismuth ferrite nanoparticles and polylactic-co-glycolic acid (PLGA) were synthesized by co-precipitation and single emission method, respectively. 3D technique was chosen to produce MNs based on Gelma which was used as the base for biodegradable and biocompatible MNs. Bare and drug loaded MNs were characterized by Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), X-Ray diffraction analysis (XRD) and Scanning Electron Microscopy (SEM). Also, in vitro studies of levetirecetam-loaded bismuth ferrite nanoparticles and phenytoin-loaded PLGA nanoparticles loaded GelMA based MN' s were then performed to evaluate drug loading. Biocompatibility testing is performed with human neuroblastoma cells and the results show that the scaffolds are biocompatible with the cells. Given the versatility of GelMA as a material for constructing tissue scaffolds, it is anticipated that 3D-printed GelMA MNs scaffold could serve as a platform for delivering epilepsy treatments.