Seventy-five non-carious premolars were randomly divided into five equal groups. The brackets were bonded with five different adhesives (Transbond XT, Eagle Bond, Light Bond, Blugloo, Unite) and subjected to artificial accelerated photoageing for 24 hours. The enamel surfaces were colourimetrically evaluated before bonding, following debonding and cleaning with a tungsten carbide bur, after polishing with Stainbuster, and after photoageing of the debonded enamel surface. The Commission Internationale de l'Eclairage(CIE) colour parameters (L*a*b*) were recorded and colour differences (delta E) were calculated. The results were statistically analyzed using the Kruskall-Wallis test. Further investigation among subgroups was performed using Dunn's multiple correlation test (P < 0.05). The clinical detection threshold for delta E value was set at 3.7 units.