Occurrence of T-2 toxin in processed cereals and pulses in Turkey determined by HPLC and TLC


Omurtag G., Yazicioglu D.

FOOD ADDITIVES AND CONTAMINANTS, cilt.18, sa.9, ss.844-849, 2001 (SCI İndekslerine Giren Dergi) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 18 Konu: 9
  • Basım Tarihi: 2001
  • Doi Numarası: 10.1080/02652030110046901
  • Dergi Adı: FOOD ADDITIVES AND CONTAMINANTS
  • Sayfa Sayıları: ss.844-849

Özet

The purpose of this study was to investigate the T-2 toxin level of contaminated cereal and pulse products in Turkey. T-2 toxin was detected using high performance liquid chromatography (HPLC) with UV detection at 208 nm and thin layer chromatography (TLC) was used for confirmation of the T-2 toxin-contaminated samples (greater than or equal to1 ppm). The recovery was 93 +/-3.3% (SD 3.29, n = 5) for chickpea spiked with a known amount of T-2 toxin (1 ppm). The detection limits for T-2 toxin for HPLC and TLC were 25 ng per injection and 50 ng per spot, respectively. A total of 50 commercially available cereal and pulse product samples, collected from markets and street bazaars, were analysed. Incidences of T-2 toxin detected in cereal and pulse products were 23.5% and 31.2%, respectively and the maximum detected amount was 1.9 ppm in a sample of dried beans. The incidence of toxin-contaminated specimens is not so low relative to the volume of specimens produced.