Akademik Veri Yönetim Sistemi
Biological and Chemical Research, sa.6, ss.8-13, 2019 (Hakemli Dergi)
: Benzoic acid (C6H5COOH) is widely used in the food industry as a preservative in acid foods, under code
number E210, owing to its antimicrobial activity against various bacteria, yeasts and fungi involved in food
poisoning and food spoilage. It is a safe preservative used to protect taste, odor, appearance, structure of food during
storage, preparation, packaging, transport and storage of food. Benzoic acid can be obtained synthetically or it can
be obtained naturally from some foods such as fruits, vegetables, spices, nuts, milk and dairy products. However,
their excessive use could lead to metabolic acidosis, convulsions, and hyperpnoea in humans. In this study, it was
aimed to obtain natural benzoic acid at the highest level in cranberry (Vaccinium macrocarpon Ait) by trying
different extraction methods such as ultrasonic extraction, orbital shaker at different durations. . Chromatographic
conditions; mobile phase: 0.1 M acetate buffer/ MeOH. Column: Kromasil C18 (5μm, 4.6 ×250 mm i.d.), Column
Temperature: 25 0
C, Detector: DAD, Wavelenght λ: 230 nm, Flow Rate: 1 mL/min., Injection Volume: 20µL. The
best result was obtained from 15 minutes of ultrasonic extraction as 30.8 ppm (mg/kg) by using methanol: water
(70:30) solution. According to this study 15 minutes of ultrasonic extraction gave better results than the 2 hour of
orbital shaker and it showed that benzoic acid’s structure was damaged by over 15 minutes of ultrasonic extraction.