Akademik Veri Yönetim Sistemi
3rd INTERNATIONAL EURASIAN MYCOLOGY CONGRESS (EMC’22), Van, Türkiye, 7 - 09 Eylül 2022, ss.129-130, (Özet Bildiri)
Mushrooms are products that have been consumed both as food and medicinally for many years. Fungi are of great economic importance and are directly and indirectly involved in maintaining the ecological balance. The enumeration, taxonomy, biology, chemistry, cultivation of mushrooms, and their conversion are of great relevance and fAŞÇInation. Wild mushrooms have become important in our diet not only owing to their nutritional value but also due to nutraceutical attributes that provide medical or health benefits to consumers. Coprinus atramentarius (Bull.) Fr. is a mushroom found in Europe and North America. The cap is bellshaped when young, becoming widespread, flattening, and fragmenting as it ages. The diameter of the cap is 3-10 cm. The color of the cap is gray-brown from the center outwards, and it is covered with brown scales. They usually occur in small groups and clusters, sometimes singly, in tree stumps or in the buried wood of deciduous hardwoods. They are also frequently seen in urban habitats such as vacant lots and lawns, and parks, often just after rains in spring and autumn. In addition, the most studied feature of C. atramentarius mushroom is the “disulfiramlike” effect on the liver when the coprin substance in its content is taken with alcohol. This has been proven by in vivo and in vitro studies. Therefore, it can also be used for the treatment of alcohol dependence. In this research, in vitro antidiabetic, antioxidant, antimicrobial activities, and anatomical properties of C. atramentarius have been evaluated. Coprinus atramentarius was collected in Bolu, Türkiye, on October 5, 2021. The mushroom was authenticated by Ilgaz AKATA. The methanolic extract (CAM) was prepared from C. atramentarius by maceration, then the main extract was solved with distilled water (CAW) and fractionated with n-hexane (CAH, dichloromethane (CAD), ethyl acetate (CAEA), and n-butanol (CAB). α-Glucosidase and α-amylase enzyme inhibitory acitivities of mushroom were evaluated for antidiabetic activity. Antioxidant activity was determined using DPPH and ABTS radical scavenging assays. Additionally, total phenolic content was determined. The antimicrobial activity of the mushroom was determined by using microdilution methods against Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6538, and Candida albicans ATCC 10231 (CLSI, 2006; CLSI, 2008). Anatomical investigations were made in a Jeol JSM 6490LV scanning electron microscope at the Turkish Petroleum International Company (TPAO) Research Centre SEM laboratory, Ankara. In antioxidant acitivity studies, CAEA extract displayed 16.02% ABTS radical scavenging effect (90.13% for α-tocopherol and 96.60% for trolox) at 60 ug/mL while 6.039% DPPH radical scavenging effect (91.96% for α-tocopherol and 93.32% for trolox) at 100 μg/mL. According to the total phenolic content (μg GAE/mg extract) was found as parallel with antioxidant studies, the total phenolic content of CAEA extract was determined as the highest one (16 µg GAE/mg extract). It was concluded that no significant inhibitory effect on αglucosidase and α-amylase enzymes was observed when compared to acarbose. It is the first evaluation on antidiabetic activity of this mushroom. In antimicrobial activity study, the CAW extract was effective with 250 μg/mL MIC value against E. coli, and the CAB extract was effective with 125 and 62.5 μg/mL MIC values against S. aureus, and C. albicans, respectively. In a previous study, the β-glucan extract of prepared from C. atramentarius displayed antioxidant and antimicrobial activities. According to anatomy studies, the shape of spore of mushroom was found as ellipse and size of spore long axis was determined as 6-7.5 μm and size of short axis was found as 3-4 μm.
KEYWORDS: Coprinus atramentarius, antioxidant, antimicrobial, antidiabetic, total phenolic, spore