An efficient separation and method development for the quantifying of two basic impurities of Nicergoline by reversed-phase high performance liquid chromatography using ion-pairing counter ions

Yalcin, Guler; Yuktas, F.

doi:10.1016/j.jpba.2006.04.032

An efficient separation and method development for the quantifying of two basic impurities of Nicergoline by reversed-phase high performance liquid chromatography using ion-pairing counter ions

Yalcin G., Yuktas F. N.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, vol.42, no.4, pp.434-440, 2006 (SCI-Expanded)

Publication Type: Article / Article
Volume: 42 Issue: 4
Publication Date: 2006
Doi Number: 10.1016/j.jpba.2006.04.032
Journal Name: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Page Numbers: pp.434-440
Marmara University Affiliated: No

Abstract

A quantification method was developed for the two basic impurities, one of which is also a metabolite, of Nicergoline (NIC), by using reversed-phase high performance liquid chromatography (RP-HPLC) and diode array detector (DAD). One of these compounds, 10-methoxy-6-methylergoline-8 beta-methanol-5-bromo-3-pyridinecarboxylate (1-DN) is the metabolite as well as the impurity whereas, the other 10-methoxy-1,6-dimethylergoline-8 beta-methanol-5-chloro-3-pyridinecarboxylate (5-CN) is only an impurity.