Aqueous garlic extract alleviates ischaemia-reperfusion-induced oxidative hepatic injury in rats


Sener G. , Sehirli O., Ipci Y., Ercan F. , Sirvanci S. , Gedik N., ...Daha Fazla

JOURNAL OF PHARMACY AND PHARMACOLOGY, cilt.57, ss.145-150, 2005 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 57 Konu: 1
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1211/0022357055209
  • Dergi Adı: JOURNAL OF PHARMACY AND PHARMACOLOGY
  • Sayfa Sayıları: ss.145-150

Özet

This study was designed to examine the effects of aqueous garlic extract (AGE) on hepatic ischaemia-reperfusion (I/R) injury in rats. For this purpose, Wistar albino rats were subjected to 45min of hepatic ischaemia, followed by a 60-min reperfusion period. AGE (1 mL kg(-1), i.p., corresponding to 500 mg kg(-1)) or saline was administered twice, 15 min before ischaemia and immediately before the reperfusion period. Serum aspartate aminotransferase (AST) and alanine aminotransferase.(ALT) levels were determined to assess liver functions. Liver tissues were taken for the determination of malondialdehyde (MDA) levels, an end product of lipid peroxidation; glutahione (GSP.) levels, a key antioxidant; and myeloperoxidase (MPO) activity, as an indirect index of neutrophil inifiltration Hepatic collagen content, as a fibrosis marker, was also determined. Plasma ALT and AST activities, were elevated in the I/R group as compared with the control group, while these increases significantly decreased by AGE treatment. Hepatic GSH levels, significantly, depressed by I/R were elevated back to control levels in the AGE-treated I/R group. Increases in tissue MDA levels and MPO activity due to I/R injury were reduced back to control levels by AGE treatment. Similarly,, increased hepatic collagen content in the I/R group was reduced to the control level with AGE treatment. Since AGE administration alleviated the I/R-incluced injury of the liver and improved the hepatic and function, it seems likely that AGE, with its antioxidant and oxidant-scavenging properties, may be of potential therapeutic value in protecting the liver against oxidative injury due to ischaemia-reperfusion.