Akademik Veri Yönetim Sistemi
Applications of Chemistry in Nanosciences and Biomaterials Engineering NanoBioMat 2024 – Summer Edition, Bucuresti, Romanya, 19 - 21 Haziran 2024, ss.101-102
Cancer is characterized by abnormal and uncontrolled cell growth resulting from genetic or epigenetic changes in somatic cells, with the ability to spread to other parts of the body. It continues to rank as the most significant health issue after cardiovascular diseases [1] . Despite significant progress in both diagnosis and treatment, the prevalence and fatality rates of cancer persistently escalate, underscoring the urgent need for innovative approaches in combating this formidable disease. The nano-oncological paradigm stands out as one of the most efficacious methodologies in contemporary cancer therapeutics. The primary objective of synthesizing poly(lactic acid) (PLA)-based nanofibers loaded with Doxorubicin, an antineoplastic chemotherapy agent, is to enhance the drug's pharmacokinetic profile. Doxorubicin possesses a relatively short elimination half-life of approximately 34.7 ± 16.6 hours [2], which may limit its therapeutic efficacy due to rapid clearance from the body. By encapsulating Doxorubicin within PLA nanofibers, the aim is to prolong its presence within the systemic circulation and ensure sustained release over an extended duration, thereby improving its therapeutic effectiveness against cancerous cells. In this study, pure and Doxorubicin loaded PLA nanofibers were synthesized by electrospinning method. Optimization of parameters such as flow rate, polymer concentration, voltage, distance was performed in fiber formation [3] . Synthesized nanofibers characterized by FTIR, XRD, DSC, SEM analysis, and cell viability against A549 lung cancer was examined by MTT assay at 24 hours. The SEM results indicate that the nanometer-scale fibers exhibit a free-beaded, continuous, and uniform structure, with an increase in fiber diameter from 372 nm to 434 nm upon Doxorubicin loading. Additionally, significant improvements were achieved in in vitro release of DOX from nanofibers in phosphate buffer solution (PBS) at pH = 7.4 and 24-h MTT results.