Akademik Veri Yönetim Sistemi
16th Horizons in Molecular Biology Göttingen-Germany, Göttingen, Almanya, 9 - 12 Eylül 2019, cilt.1, ss.127
Many cells and tissue express a mixture of cholinergic
receptors. Different studies have suggested that nonneuronal acetylcholine and
cholinergic agonists alter cell growth and proliferation of T and B lymphocytes.
Costa et al. demonstrated that acetylcholine released from T-lymphocytes acts
via the M3 acetylcholine muscarinic receptor (mAChR) to
trigger signaling pathways. Muscarinic cholinergic receptors activate stimulatory growth mechanisms
in megakaryocytic differentiated K562 leukemia cells.
In this study, we investigated the levels of
caspase 3,8 and 9 protein expression and
caspase activity in megakaryocytic differentiated K562 leukemia cells.
And we also investigated the effects of muscarinic agonist effects
on apoptosis megakaryocytic differentiated K562 leukemia cells.
Megakaryocytic differentiation was induced
by applying phorbol 12-myristate 13-acetate (PMA) (Calbiochem) at the
concentration of 10 nM. Anti-Caspase 3,8 and 9 antibodies were purchased from
Santa Cruz, Inc. .). For western blot assay, Proteins were electrotransferred
to nitrocellulose membranes which were exposed to primary antibodies and then
to alkaline phosphatase-conjugated secondary antibodies. The antibody–antigen
complex was detected with NBT/BCIP. Caspase activity detected by Elisa technique.
We
detected caspase 3,8 and 9 activity and expression in megacaryocytic
differentiated K562 leukemia cells. Especially muscarinic receptor agonist and
antagonist decrease caspase 3 and caspase 9 expression. These results suggest that CCh may be modulates megakaryocytıc
dıfferentiated K562 leukemic cells apoptosis through muscarinic
acetylcholine receptors