Fabrication of Amphotericin-B-loaded Sodium Alginate Nanoparticles for Biomedical Applications

ULAĞ S., Celik S. E., ŞENGÖR M., GÜNDÜZ O.

BIONANOSCIENCE, cilt.12, sa.4, ss.1230-1237, 2022 (ESCI) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 12 Sayı: 4
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s12668-022-01018-5
  • Dergi Adı: BIONANOSCIENCE
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, Compendex, EMBASE, INSPEC
  • Sayfa Sayıları: ss.1230-1237
  • Anahtar Kelimeler: Amphotericin-B, Biomedical, Drug release, Electrospraying, Nanoparticle, Sodium alginate
  • Marmara Üniversitesi Adresli: Evet

Özet

In this study, amphotericin-B (AMB)-loaded sodium alginate (SA) nanoparticles were fabricated using the electrospraying technique for biomedical applications. AMB is an antifungal agent and is poorly absorbed from the gastrointestinal tract due to its low aqueous solubility. Therefore, it should be given parenterally to treat systemic fungal infections. This study aims to transport it with nanoparticle formulations and observe the nanoparticle release behaviours. Scanning electron microscopy (SEM) images showed that nanoparticles of 0.5% SA fabricated at 37 kV had the most suitable particle diameter (93.36 +/- 24.386 nm) for loading 0.5, 1, and 3 ml of AMB. Fourier transform infrared spectroscopy (FTIR) results demonstrated that AMB successfully loaded into 0.5% SA nanoparticles. Drug release behaviours of the AMB-loaded particles indicated that AMB was released with a burst at the beginning, and release behaviour became sustainable after half an hour. The encapsulation efficiencies of the different amounts of drug were calculated, and the results showed that the highest encapsulation efficiency belonged to the 0.5% SA/1 AMB nanoparticles (42 +/- 1.23%).