Evaluation of isolated and combined effects of riluzole and sodium valproate in genetic absence epilepsy rats

Karamahmutoglu T., Sahin E., Gurbuz B., Sukurov T., Gunes A. N., Faki E., ...Daha Fazla

Marmara Medical Journal, cilt.38, sa.1, ss.68-75, 2025 (ESCI, Scopus, TRDizin)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38 Sayı: 1
  • Basım Tarihi: 2025
  • Doi Numarası: 10.5472/marumj.1629028
  • Dergi Adı: Marmara Medical Journal
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, Academic Search Premier, CINAHL, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.68-75
  • Anahtar Kelimeler: Absence epilepsy, Anti-seizure, Ethanol, GAERS, Riluzole, Sodium valproate
  • Marmara Üniversitesi Adresli: Evet

Özet

Objective: In epilepsy treatment, drugs that increase GABAergic and decrease glutamatergic activity, like sodium valproate, are used. Riluzole is a neuroprotective drug that blocks glutamatergic neurotransmission and is a potential drug with its anti-seizure effects. This study evaluates the anti-seizure effects of sodium valproate and riluzole on the genetic absence epilepsy rat from Strasbourg (GAERS) model individually and combined. Materials and Methods: Adult male GAERS rats (n = 22) were used. Rats were administered 5/10 mg/kg riluzole, 150/300 mg/kg sodium valproate, 95% ethanol, and 5 mg/kg riluzole combinations with 150-300 mg/kg sodium valproate intraperitoneally. EEG recordings and locomotor activity tests were conducted. Statistical analysis was performed using GraphPad Prism. Results: Post-injection of 10 mg/kg riluzole significantly decreased the number of spike-wave-discharges (SWDs) (p = 0.04) compared to the control group. A synergistic effect was observed with 5 mg/kg riluzole and 150 mg/kg sodium valproate, reducing total seizure time (p = 0.03) and SWDs (p = 0.03). Conclusion: The study demonstrated the anti-seizure effects of 150/300 mg/kg sodium valproate, 10 mg/kg riluzole, and ethanol. A synergistic effect of 5 mg/kg riluzole with 150 mg/kg sodium valproate was noted. As an isolated or combined solution, riluzole shows potential, especially in resistant epilepsy treatment.