Akademik Veri Yönetim Sistemi
Advances in Health and Natural Sceinces, Dr. Burcu Yüksel,Dr. Mustafa Sencer Karagül, Editör, NOVA Science Publishers Inc. , New-York, ss.203-222, 2018
The transmission of the energy to the higher levels is through the
food chains, starting from the main source plants. There is a balance
between all the livings in the ecosystem in which the plants are the first
ring of this chain. Environmental mutagens and other related substances
threaten humans and all other living organisms in an ecosystem. Plants
are exposed to various types of environmental agents such as agricultural
pesticides and plant growth regulators, or accidentally present in polluted air, soil or water. While pesticides used against harmful effects of
agricultural production affect the target organisms, they can affect the
non-target organisms through wrong and especially excessive
applications. The adverse effects of toxic agents that accumulate in
aquatic and terrestrial crops in an ecosystem and accumulate there as a
bioindicator in plants should be identified?. Plant biotests are very
important methods for evaluating the genotoxic contamination in the
environment. For quick determination and measurement of the
genotoxicity for these stress factors various molecular and biochemical
tests are required. Single cell gel electrophoresis is also known as Comet
assay that is used to investigate the cell response to several biotic and
abiotic genotoxic agents leading to oxidative DNA damage. The Comet
assay is used as a useful tool in assessing the potential of plants as source
of information on the genotoxic impact of dangerous pollutants and
sensitive sensors in ecosystems. This technique is based on the
quantification of denatured DNA fragments migrating out of the cell
nucleus during electrophoresis. Current review aims to give information
about Comet assay and its applications as a valuable tool for monitoring
DNA damage in different plant systems. The single cell gel
electrophoresis studies were performed by our group to determine the
genotoxic effects of different concentrations of pesticides, heavy metals
and different plant growth regulators used in tissue culture applications in
tobacco as model organisms, cereals (barley and wheat) and sunflower.