Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, cilt.30, sa.2, ss.188-195, 2020 (Hakemli Dergi)
Aim: This study aimed to evaluate the antibacterial and antifungal effects of different disinfection methods for infected root canals with Enterococcus faecalisor Candida albicans in vitro. Material and Methods:Hundred extracted, single-rooted human teeth with straight root canals wereselected. After chemo-mechanical preparation of the root canals, except of thosein the negative control group, contamination was performed using Enterococcus faecalisor Candida albicans for 7 days. The infected teeth were divided into three subgroups (n=15) according to the disinfection method applied: photo‑activated disinfection, photon-induced photoacoustic streaming and 2.5% sodium hypochlorite irrigation. Microbial samples were collected from the root canals before and after disinfection.Ahundred colony forming units were counted, and data were statistically analysed.Results: All experimental groups showed significant reduction of Enterococcus faecalisand Candida albicans. Photon-induced photoacoustic streaming and sodium hypochloriteirrigation had similar antimicrobial effect, which was higher than photo‑activated disinfectionfor both microorganisms (p<0.05). Conclusion: None of the testing methods for root canal disinfection was able to achieve complete elimination of microorganisms. However, the results of this study state that photon-induced photoacoustic streaming, photo‑activated disinfection and 2.5% NaOCl has significant antimicrobial effectagainst Enterococcus faecalisand Candida albicansbiofilms in root canals.