Akademik Veri Yönetim Sistemi
Applications of Chemistry in Nanosciences and Biomaterials Engineering NanoBioMat 2023 – Summer Edition, Bucuresti, Romanya, 26 - 30 Haziran 2023, ss.114-115
Tendon tissue engineering aims to integrate engineered, functional replacements with their native
counterparts in order to repair tendon injuries. Responsible for body movement and stabilization of the joint
structure, tendons stand out with their superior biomechanical properties. Collagen 1 (COL) is the main
component that contributes these properties to the tendon structure organized in hierarchical bundles.
Hyaluronic acid (HA) participates in the formation of the tendon extracellular matrix and enhances the
viscoelasticity of the structure. Polylactic acid (PLA) is widely used in tendon tissue engineering due to its
biocompatibility, biodegradability and mechanical properties. Therefore, a novel PLA/HA/COL hybrid
scaffold was designed and fabricated by electrospinning for the repair of tendon defects. While 10% PLA
is used as the control group, 4 scaffolds containing 1% HA and/or 1% COL were fabricated. One of the
scaffolds was produced by coaxial electrospinning: the inner layer consists of 10% PLA and 1% COL, while
the composition of the outer layer is 10% PLA and 1% COL. The morphological, thermal, chemical, and
mechanical properties of the tendon scaffolds were evaluated using SEM, DSC, FT-IR, swellingdegradation test, and tensile testing test, respectively. In vitro cell culture experiments were performed on
mesenchymal stem cells using MTT method. It is anticipated to confirm that PLA/HA/COL scaffolds
promote cell adhesion, proliferation, and regeneration by characterisation and in vitro cell culture results.