Effect of BCG accination on cytokine mRNA expression in atopic children with asthma

Ozera A., Tukenmezb F., Biricika A., Barlanb I., Cirakoglua B., Basarana M.

IMMUNOLOGY LETTERS, cilt.86, sa.1, ss.29-35, 2003 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 86 Sayı: 1
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1016/s0165-2478(02)00261-4
  • Dergi Adı: IMMUNOLOGY LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.29-35
  • Anahtar Kelimeler: BCG, atopy, asthma, CALMETTE-GUERIN, MICE, INTERLEUKIN-12, INFECTION, OVALBUMIN, VACCAE
  • Marmara Üniversitesi Adresli: Evet

Özet

Background: To inestigate whether a preexisting TH2-type immune response could be suppressed by BCG immunization in atopic children with asthma. Methods and results: We hae used PCR to amplify reerse transcribed RT IFN- and IL-5 mRNA expressed by peripheral blood mononuclear cells PBMCs in response to in itro phytohemagglutinin A, purified protein deriatie and Dermatophagoides pteronyssinus II stimulation from nine atopic children, both before and 8 weeks after BCG accination. We hae demonstrated that IFN- expression was induced in response to all stimulants IFN-actin after the accination, whereas there was no expression before P<0.001. Although there was a tendency to diminish in the expression of IL-5 mRNA in response to the stimulants, only PHA rendered a statistically significant decrease after the accination. Conclusions: These results proide some eidence of TH1 dominance after BCG administration in atopic children. 2002 Elseier Science B.V. All rights reserved.