Effect of Horse-chestnut seed extract on matrix metalloproteinase-1 and-9 during diabetic wound healing

Aksoy H. , ÇEVİK Ö., ŞEN A. , Goger F., ŞEKERLER T. , ŞENER A.

JOURNAL OF FOOD BIOCHEMISTRY, vol.43, no.3, 2019 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 43 Issue: 3
  • Publication Date: 2019
  • Doi Number: 10.1111/jfbc.12758
  • Keywords: diabetic wound, Horse chestnut seed extract, LC-MS/MS, matrix metalloproteinase-1, matrix metalloproteinase-9, wound healing, AESCULUS-HIPPOCASTANUM L., PHENOLIC-COMPOUNDS, OXIDATIVE STRESS, LEAF TISSUES, ANTIOXIDANT, EXPRESSION, QUERCETIN, EFFICACY, INHIBITOR, AESCIN


The effects of aqueous-ethanol extract of Horse chestnut (HCE) on MMP-1 and MMP-9 expressions during cutaneous wound healing in diabetic rats were investigated in this study. The expressions of MMP-1 and MMP-9, wound closure, myeloperoxidase (MPO) activity, hydroxyproline, and malondialdehyde (MDA) levels in wound tissue were measured. Quercetin glucuronide in HCE was identified as main compound using a LC-MS/MS. The hydroxyproline level was significantly increased in the treated group versus control after the 3rd and 7th days (p < 0.05). The MDA level and MPO activity were significantly lower in the treatment group (p < 0.05). MMP-1 gene expression level in treated rats was increased in the 7th day while it was reduced in 14th day. MMP-9 gene expression level in treated rats was decreased in 7th, and 14th days compared to control (p < 0.05). These results show that HCE accelerated the cutaneous wound-healing process in diabetic rats via MMP-1 and MMP-9 regulation.