Evaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the identification of mycobacteria species

Akyar I., ÇAVUŞOĞLU C., Ayas M., SÜRÜCÜOĞLU S., Ilki A., Kaya D. E., ...Daha Fazla

TURKISH JOURNAL OF MEDICAL SCIENCES, cilt.48, sa.6, ss.1351-1357, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 48 Sayı: 6
  • Basım Tarihi: 2018
  • Doi Numarası: 10.3906/sag-1801-198
  • Dergi Adı: TURKISH JOURNAL OF MEDICAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.1351-1357
  • Anahtar Kelimeler: MALDI-TOF MS, identification, Mycobacterium, Mycobacterium tuberculosis complex, nontuberculous mycobacteria, DESORPTION IONIZATION-TIME, FLIGHT MASS-SPECTROMETRY, SP NOV., SUBSP ABSCESSUS, DISEASE, INFECTION, CULTURES, CHIMERA, RELAPSE, MEMBERS
  • Marmara Üniversitesi Adresli: Evet

Özet

Background/aim: Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is an alternative way of identifying mycobacteria via the analysis of biomolecules. It is being increasingly used in routine microbiology practice since it permits early, rapid, and cost-effective identification of pathogens of clinical importance. In this study, we aimed to evaluate the efficacy of phenotypic identification of mycobacteria by the MALDI-TOF MS MBT Mycobacteria Library (ML) 4.0 (Bruker, Daltonics) compared to standard sequence analysis.