Akademik Veri Yönetim Sistemi
REVISTA ROMANA DE MATERIALE/ ROMANIAN JOURNAL OF MATERIALS, cilt.53, sa.3, ss.189-204, 2023 (SCI-Expanded)
Diabetic wounds pose a significant challenge in clinical settings, primarily due to uncontrolled blood sugar levels and
compromised immune systems observed in diabetes mellitus. Curcumin (CUR), a well-known anti-inflammatory and antioxidant
agent, has shown potential for wound regeneration. However, its limited bioavailability and stability necessitate the development
of a novel scaffold to overcome these limitations. In this study, we investigated the wound-healing properties of extracted CUR
from Curcuma longa rhizomes and their combination with organic polymers by developing and evaluating a biomimetic composite
3D scaffold specifically designed for diabetic wounds. Incorporating CUR and Ethyl cellulose (EC) nanoparticles onto Alginate
and Chitosan (SA/CS) scaffolds was achieved through the electrospraying method, resulting in an average particle size of 784±35.1
nm for CUR/EC nanoparticles. The interaction between different concentrations of CUR1 and CUR3 (1mg/1ml and 3mg/1ml) with
EC on scaffolds was analyzed using FTIR spectroscopy, providing insights into the chemical composition of the scaffolds. The
structural properties of the scaffolds and nanoparticles were examined to assess their morphological characteristics.
Furthermore, the swelling and degradation behaviors of the scaffolds were investigated under in vitro conditions. The release
profile of CUR was determined, and cytotoxicity testing was performed on the L929 cell line using an MTT assay to evaluate the
biocompatibility of CUR/EC-coated scaffolds, as indicated by viable and proliferating cells in vitro study. In addition, the potential
of CUR/EC-coated scaffolds to support tissue regeneration was evaluated using a DAPI staining assay and scanning electron
microscopy (SEM).