The effect of polycaprolactone/graphene oxide electrospun scaffolds on the neurogenic behavior of adipose stem cells

Pinar E., Sahin A., Unal S., Gunduz O., Harman F., Kaptanoglu E.

EUROPEAN POLYMER JOURNAL, cilt.165, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 165
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.eurpolymj.2022.111000
  • Dergi Adı: EUROPEAN POLYMER JOURNAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Chemical Abstracts Core, Communication Abstracts, INSPEC, Metadex, Civil Engineering Abstracts
  • Anahtar Kelimeler: Adipose-derived stem cell, Graphene oxide, Polycaprolactone, Nanofiber scaffold, Neuronal differentiation, Electrospinning, GRAPHENE-BASED MATERIALS, HYDROGEL SCAFFOLDS, DIFFERENTIATION, TISSUE, THERAPY, GROWTH, PCL
  • Marmara Üniversitesi Adresli: Evet

Özet

Stem cell destiny can be controlled with scaffold biomaterials in tissue engineering and regenerative medicine. This study aimed to investigate the neuronal differentiation potential of human adipose tissue-derived mesenchymal stem cells in graphene nanofiber matrix in vitro. Stem cell isolation was performed from adipose tissue taken from human by mechanical and enzymatic methods. The differentiation potential was examined after incubation of adipose stem cells in normal medium and neural differentiation medium, on graphene oxide (GO) and polycaprolactone (PCL) composite scaffolds produced by electrospinning technique. In vitro studies indicated that the presence of GO in PCL scaffold increases an effect on cell attachment, proliferation, infiltration into the scaffold, and neuronal differentiation. Also, unlike subcutaneous tissue, it has been shown immunohistochemically that mesenchymal stem cells derived from epidural adipose tissue tend to differentiate into oligodendrocytes.