Niğde Ömer Halisdemir University Journal of Engineering Sciences, cilt.14, sa.1, ss.1-9, 2025 (Hakemli Dergi)
Spectrophotometers are a kind of photometers that are used to find the
amount of substance in the solution. Spectrophotometers detect the
amount of radiation that is not absorbed by the solution in the sample
chamber and gives information about the amount of substance in the
sample. Spectrophotometers are used in many different fields such as
chemistry, medicine, geology, environmental engineering and agriculture.
Samples that will directly affect people, such as wastewater, drinking
water, pharmaceuticals, are analyzed with high accuracy and possible
hazards are avoided. In this study, a device that can be used in the
detection of Salmonella bacteria is modeled. In addition, the fact that
the heater unit of the device is on the test mechanism eliminates the
need for heating in the external environment and the test data can be
saved in the cloud environment over the internet connection, which is
the novelty of this study. A spectrophotometer device was developed
using Raspberry Pi 3 and Python software language. A 470 nm optical
filter was used in the device. In addition to the features of standard
spectrophotometers, the heating feature that can keep the sample
temperature at the desired value has been added. This feature has been
developed using the on-off logic method. Linear regression of optical
measurements, R2 value was calculated as 0.9982 in density tests between
0.1 gram and 0.2 gram. The device was developed with a Raspberry Pi 3
minicomputer, it does not need an external computer for use. Thanks to
the internet connection ability of Raspberry Pi 3, the analysis results
are transferred to the cloud environment, allowing the user to see the
results even when they are not at the device.