Role of the Nitric Oxide on Relaxation of the Human Umbilical Artery during Cooling


Atalik K. E. N. , NURULLAHOĞLU Z. U. , KILIÇ M.

YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, cilt.131, sa.5, ss.661-667, 2011 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 131 Konu: 5
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1248/yakushi.131.661
  • Dergi Adı: YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN
  • Sayfa Sayıları: ss.661-667

Özet

In the present study, the effects of cooling (to 28 degrees C) on the vasodilatation induced by diazoxide (10(-9)-3x 10(-4) M), isoproterenol (10(-9)-3x 10(-4) M) and magnesium sulphate (0.1-30 mM) on serotonin-pre-contracted human umbilical artery and the role of nitric oxide in these effects were analyzed. Diazoxide, isoproterenol and magnesium produced concentration-dependent relaxation of human umbilical artery precontracted with serotonin (10(-6) M). During cooling, the pIC(50) values and maximal responses to these agents were significantly lower than at 37 degrees C. Cooling to 28 degrees C in the presence of N-G-nitro-L-arginine methyl ester (L-NAME, 10(-4) M) did not modify the effects of temperature on diazoxide, isoproterenol and magnesium-induced relaxations. These results suggest that cooling-induced changes of diazoxide, isoproterenol, and magnesium sulphate in human umbilical artery are independent of nitric oxide.