Carbapenem-hydrolyzing oxacillinase, OXA-48, persists in Klebsiella pneumoniae in Istanbul, Turkey

Aktaş Z., Kayacan Ç. B., Schneider I., Can B., Midilli K., Bauernfeind A.

Chemotherapy, cilt.54, sa.2, ss.101-106, 2008 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 54 Sayı: 2
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1159/000118661
  • Dergi Adı: Chemotherapy
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.101-106
  • Anahtar Kelimeler: Carbapenemase, Klebsiella pneumoniae, OXA-48, Oxacillinase
  • Marmara Üniversitesi Adresli: Hayır

Özet

Background: Two OXA-48-producing Klebsiella pneumoniae isolates(KP-4936 and KP-154488) were analyzed. Method: Minimum inhibitory concentrations were determined using agar dilution and E-test, β-lactamase production by phenotypic tests (E-test MBL and ESBL, isoelectric focusing, and bioassay) and molecular methods (PCR, RAPD-PCR, sequencing, plasmid analysis, and conjugation). Results: Isolateswere resistant to all β-lactams, including carbapenems. PCR and sequencing identifiedblaOXA-48in bothisolates and the transconjugant. KP-4936 harbored blaTEM-1 (pI 5.4) and blaCTX-M-15 genes (pI 8.6), while KP-154488 was positive for bla TEM-1 (pI 5.4), blaCTX-M-15 (pI 8.9), and bla SHV2a (pI 7.6), in addition. The enzyme with a pI of 7.2 hydrolyzed imipenem according to a bioassay result. Plasmids (70 and 140 kb) from KP-4936 were transferred by conjugation. RAPD-PCR found no clonal relationship between the two strains. Conclusion: Carbapenem resistance may spread among Enterobacteriaceaevia the transferable enzyme OXA-48. Copyright © 2008 S. Karger AG.