Production of a high molecular weight levan by Bacillus paralicheniformis, an industrially and agriculturally important isolate from the buffalo grass rhizosphere

Nasir A., Ahmad W., Sattar F., Ashfaq I., Lindemann S. R., Chen M., ...Daha Fazla

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, cilt.115, sa.9, ss.1101-1112, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 115 Sayı: 9
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s10482-022-01760-6
  • Dergi Adı: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1101-1112
  • Anahtar Kelimeler: Bacillus paralicheniformis, Exopolysaccharide, Fructan, Fructooligosaccharide, Levan, STRUCTURAL-CHARACTERIZATION, ANTITUMOR-ACTIVITY, FRUCTANS, INULIN, FRUCTOOLIGOSACCHARIDES, LEVANSUCRASE, PREBIOTICS, PHYLOGENY, GROWTH
  • Marmara Üniversitesi Adresli: Evet

Özet

A new exopolysaccharide (EPS) producing Gram-positive bacterium was isolated from the rhizosphere of Bouteloua dactyloides (buffalo grass) and its EPS product was structurally characterized. The isolate, designated as LB1-1A, was identified as Bacillus paralicheniformis based on 16S rRNA gene sequence and phylogenetic tree analysis. The EPS produced by LB1-1A was identified as a levan, having beta(2 -> 6) linked backbone with beta(2 -> 1) linkages at the branch points (4.66%). The isolate LB1-1A yielded large amount (similar to 42 g/l) of levan having high weight average molecular weight (Mw) of 5.517 x10(7) Da. The relatively low degree of branching and high molecular weight of this levan makes B. paralicheniformis LB1-1A a promising candidate for industrial applications.