Akademik Veri Yönetim Sistemi
Journal of Research in Pharmacy, cilt.30, sa.3, ss.933-944, 2026 (ESCI, Scopus, TRDizin)
The Lamiaceae family comprises a large number of aromatic plant species with notable medicinal, culinary, and cosmetic applications. Thymus L. genus, that belongs to Lamiaceae family, widely used in Turkish folk medicine. Thymus cilicicus taxa has been utilized in traditional medicine against various chronic disorders including, diabetes and hypertension. Previous studies have reported antioxidant, antimicrobial, antidiabetic, cytotoxic, and acetylcholinesterase (AChE) inhibitory properties in T. cilicicus extracts obtained with organic solvents. Bioactivity of the extracts were mainly associated with their phenolic compound content. However, existing literature has not yet addressed the biological activity and chemical composition of extracts obtained through traditional preparation methods such as infusion or maceration with water. The present study investigates antioxidant capacity via 1,1-diphenyl-2-picrylhydrazyl radical scavenging (DPPH), ferric reducing antioxidant potency (FRAP) and cupric reducing antioxidant capacity (CUPRAC) assays along with AChE inhibitory activities of T. cilicicus extracts (ethanol maceration, water maceration, and infusion). The extracts were also subjected to total phenolic content (TPC) and quantitative LC-HRMS analysis. LC-HRMS analysis of the tested extracts marked fumaric acid, p-coumaric acid, sinapinic acid, and caffeic acid as major compounds. Ethanol maceration extract exhibited the highest total phenolic content and antioxidant activity in DPPH radical scavenging and CUPRAC assays, while infusion extract exhibited the highest activity in FRAP assay. None of the tested extract showed AChE inhibition at 100 µg/mL.