Co-occurrence of nitrogen-converting organisms in full-scale treatment plants

Yapsakli K.

JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING, cilt.45, sa.9, ss.1060-1070, 2010 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 9
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1080/10934529.2010.486330
  • Dergi Adı: JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1060-1070
  • Anahtar Kelimeler: Ammonia and nitrite oxidizing bacteria, ammonia oxidizing archaea, Anammox, ANAEROBIC AMMONIUM OXIDATION, WATER TREATMENT PLANTS, 16S RIBOSOMAL-RNA, GRADIENT GEL-ELECTROPHORESIS, NITRITE-OXIDIZING BACTERIA, REAL-TIME PCR, ACTIVATED-SLUDGE, MICROSCALE DISTRIBUTION, QUANTITATIVE-ANALYSES, NITRIFYING BACTERIA
  • Marmara Üniversitesi Adresli: Evet

Özet

This study provides insights into nitrogen-converting microorganisms in three full-scale wastewater treatment plants (WWTPs), which were investigated and monitored according to their nitrification performance and the presence of ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB), ammonia-oxidizing archaea (AOA), and Anammox bacteria based on different molecular tools: fluorescent in situ hybridization (FISH), slot-blot hybridization, and quantitative real-time PCR. In situ hybridization clearly showed the Nitrosomonas species as the prevailing AOB, and Nitrospira-related species as the dominant NOB. Real-time PCR results exposed the Istanbul Pasakoy, Bursa West, and Bursa East WWTPs as harboring diverse nitrogen-converting microbial communities that include AOB, NOB, AOA, and Anammox bacteria. Nitrospira species had the highest fraction of nitrogen-converting organisms, which was up to 39.3% in the WWTPs throughout the two-year monitoring period. This study is the first molecular analysis of the simultaneous occurrence of these microorganisms.