The role of cyclooxygenase inhibition in the effect of alpha-melanocyte-stimulating hormone on reactive oxygen species production by rat peritoneal neutrophils

Oktar B., Yuksel M., Alican I.

PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, cilt.71, sa.1, ss.1-5, 2004 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 71 Sayı: 1
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1016/j.plefa.2003.11.009
  • Dergi Adı: PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1-5
  • Anahtar Kelimeler: alpha-melanocyte stimulating hormone, neutrophils, reactive oxygen species, chemiluminescence, NITRIC-OXIDE SYNTHASE, RECEPTORS, CHEMOTAXIS, GENERATION, MODULATION, EXPRESSION, AGONISTS, MSH
  • Marmara Üniversitesi Adresli: Evet

Özet

The effect of alpha-MSH on reactive oxygen species (ROS) production by rat peritoneal neutrophils and the effect of cyclooxygenase (COX) inhibition were investigated using the chemiluminescence (CL) technique. Cells were obtained by peritoneal lavage 4 h after administration of oyster glycogen to rats and were stimulated with lipopolysaccharide (LPS) from Salmonella enderitidis and phorbol 12-myristate 13-acetate (PMA). The increasing concentrations of alpha-MSH (10(-12)-10(-6) M) were added to stimulated cells alone or along with the COX inhibitors indomethacin, ketorolac or nimesulide (10(-8)-10(-5) M). Luminol and lucigenin CL levels were significantly increased in cells stimulated with LPS and PMA compared to unstimulated ones. alpha-MSH significantly reduced lucigenin CL values and this effect was completely reversed in the presence of indomethacin (10(-8) and 10(-7) M). In conclusion, alpha-NISH inhibits the production of superoxide radicals by activated rat peritoneal neutrophils and COX contributes to this effect. (C) 2004 Elsevier Ltd. All rights reserved.