Performance of "RESIST-3 O.K.N. K-SeT" immunochromatographic assay for the detection of OXA-48 like, KPC, and NDM carbapenemases in Klebsiella pneumoniae in Turkey.

Sağıroğlu P., Hasdemir U., Altınkanat G., Aksu B., Karatuna O., Söyletir G.

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], cilt.49, sa.4, ss.885-890, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 49 Sayı: 4
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1016/j.bjm.2018.02.002
  • Dergi Adı: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.885-890
  • Anahtar Kelimeler: Klebsiella pneumoniae, Immunochromatographic test, NDM, KPC, OXA-48, RAPID DETECTION, ESCHERICHIA-COLI, PSEUDOMONAS-AERUGINOSA, 1ST REPORT, ENTEROBACTERIACEAE, IDENTIFICATION, ISTANBUL, STRAINS, OXA-181, ISOLATE
  • Marmara Üniversitesi Adresli: Evet

Özet

In this study, the performance of the "RESIST-3 O.K.N. K-SeT" (Cofis BioConcept, Gembloux, Belgium) immunochromatographic assay was evaluated in 132 Klebsiella pneumoniae comprising 102 carbapenem resistant and 30 carbapenem susceptible isolates. Genotypically known isolates of Gram negative bacteria (n = 22) including various species were also tested by the assay as controls. The isolates tested by the immunochromatographic assay and also were run PCR for bla(KPC), bla(VIM) bla(NDM), and bla(OXA-48). The rates of bla(NDM), bla(OXA-48), and bla(KPC) in carbapenem resistant isolates were found at 52.9%, 39.2%, and 2.0%, respectively. Both bla(NDM) and bla(OXA-48) were found in six (5.9%) isolates. The results of the assay showed 100% concordance with those obtained by PCR in 132 K. pneumoniae. The agreement between the two methods was found to be identical at the isolate level. The assay also correctly detected all genotypically known isolates of Escherichia coli, Serratia marcescens, Citrobacter freundii, Enterobacter cloacae, K. pneumoniae carrying bla(KPC), bla(NDM), and/or bla(OXA-48). On the other hand, the assay did not exhibit any cross-reaction in control isolates harboring bla(IMP) and bla(VIM). We conclude that the RESIST-3 O.K.N. K-SeT is a reliable, rapid, and user friendly test and we recommend it for routine diagnostic laboratories. (C) 2018 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.