Xenin-25 improves indomethacin-induced acute gastric injury in rats
Pflugers Archiv European Journal of Physiology, cilt.478, sa.6, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 478 Sayı: 6
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s00424-026-03186-9
- Dergi Adı: Pflugers Archiv European Journal of Physiology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, EMBASE, MEDLINE, Academic Search Ultimate (EBSCO), Natural Science Collection (ProQuest), Biological Science Database (ProQuest), Biomedical Reference Collection: Corporate Edition (EBSCO), Health Research Premium Collection (ProQuest), Pharma Collection (ProQuest)
- Anahtar Kelimeler: Apoptosis, Gastric ulcer, Inflammation, Vagal afferent fibers, Xenin-25
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Marmara Üniversitesi Adresli: Evet
Özet
Putative gastroprotective effects of xenin-25 were investigated in an indomethacin-induced acute ulcer model. Male Sprague-Dawley rats were randomly assigned to control, saline-treated ulcer, xenin-25-treated ulcer groups. Gastric ulcer was induced with indomethacin (25 mg/kg, subcutaneously) and xenin-25 (0.2, 2, 20 µg/kg) or saline was given subcutaneously immediately after and at 2 h following indomethacin injection. In order to investigate whether the effects of xenin-25 depend on vagal afferent fibres, two additional groups underwent vagal afferent denervation (VAD) by bilateral perivagal capsaicin application (1%). Following a 2-week recovery, rats with VAD were induced with ulcer, after which they received either xenin-25 (2 µg/kg) or saline. All animals were sacrificed 4 h after ulcer induction, and gastric tissues were collected for biochemical, molecular, and histopathological analyses. Indomethacin administration resulted in significant increases in malondialdehyde and myeloperoxidase levels, accompanied by glutathione depletion, upregulation of nuclear factor-κB and Bax, and downregulation of Bcl2 expression. Xenin-25 treatment markedly attenuated oxidative stress, inflammatory responses, apoptotic markers, and histopathological gastric damage. Xenin-25-induced reductions in lesion length, malondialdehyde, and myeloperoxidase levels were abolished in VAD rats, but all the other anti-inflammatory and anti-apoptotic effects of Xenin-25 were preserved even in the absence of vagal afferents. Xenin-25 exhibits gastroprotective effects in acute gastric injury through its antioxidant, anti-inflammatory, and anti-apoptotic actions, which are independent of vagal afferent fibers.