Akademik Veri Yönetim Sistemi
Journal of Periodontal Research, cilt.59, sa.5, ss.993-1004, 2024 (SCI-Expanded)
Aim: The present study aimed to investigate the histomorphometric and immunohistochemical impacts of vitamin K2 on guided bone regeneration (GBR) in calvarial critical-size defects (CSDs) in diabetic rats. Methods: A total of 30 rats were used in this study, comprising 12 non-diabetic (control) rats and 18 with streptozotocin-nicotinamide-induced experimental Diabetes mellitus (DM). In all rats, two calvarial CSDs were created: one defect was left empty (E), the other was treated with bovine-derived bone graft and collagen-based resorbable membrane (GM). Study groups were as follows: control rats administered saline (n = 6, C-E and C-GM groups) or vitamin K2 (n = 6, CK-E and CK-GM groups) and diabetic rats administered saline (n = 6, DM-E and DM-GM groups) or vitamin K2 (n = 6, DMK-E and DMK-GM groups). After 4 weeks of saline or vitamin K2 administration, the rats were euthanized. Bone defect healing and new bone formation were assessed histomorphometrically, and osteocalcin and osteopontin levels were examined immunohistochemically. Results: Percentage of new bone formation was greater in CK-GM vs. CK-E and in DMK-GM vs. DMK-E [d = 3.86 (95% CI = 16.38–28.61), d = 1.86, (95% CI = 10.74–38.58), respectively, p <.05]. Bone defect healing scores were higher in CK-GM vs. CK-E and in DMK-GM vs. DMK-E [d = 2.69 (95% CI = -2.12 to −0.87), d = 3.28 (95% CI = 0.98–1.91), respectively, p <.05]. Osteocalcin expression levels were elevated in CK-GM vs. CK-E, in DMK-GM vs. DMK-E [d = 1.19 (95% CI = 0.08–1.41), d = 1.10 (95% CI = 0.02–1.22), respectively p <.05]. Vitamin K2 enhanced osteocalcin expression levels in DMK-E vs. DM-E [d = 2.78, (95% CI = 0.56–1.53), p <.05] and in DMK-GM vs. DM-GM [d = 2.43, (95% CI = 0.65–2.10), p <.05]. Osteopontin expression was enhanced in defects treated with GM vs. E defects [C-GM vs. C-E, d = 1.56 (95% CI = 0.38–2.01); CK-GM vs. CK-E, d = 1.91 (95% CI = 0.49–1.72); DM-GM vs. DM-E, d = 2.34 (95% CI = -1.12 to −0.50); DMK-GM vs. DMK-E, d = 2.00 (95% CI = 0.58–1.91), p <.05]. Conclusion: The research findings suggest that administering vitamin K2 in GBR for rats with DM favorably impacts bone healing in CSDs, presenting an adjunctive strategy for bone regeneration.