The role of epidermal growth factor and cholinergic receptor agonists and antagonists in MAPK signal transduction in K562 cells

Atmaca S. G., Aydın Omay B., Cabadak H.

MARMARA MEDICAL JOURNAL, cilt.1, sa.37, ss.86-91, 2024 (ESCI) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1 Sayı: 37
  • Basım Tarihi: 2024
  • Doi Numarası: 10.5472/marumj.1379868
  • Dergi Adı: MARMARA MEDICAL JOURNAL
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, Academic Search Premier, CINAHL, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.86-91
  • Marmara Üniversitesi Adresli: Evet

Özet

Objective: Muscarinic receptors (M1-M5) are members of the G protein-coupled receptor superfamily and are effective in physiological functions through G proteins. Recent studies suggested that cholinergic receptors mediate cellular activities in hematopoietic cells. The aim of this study was to investigate the potential role of mitogen-activated protein kinases (MAPK) signaling extracellular signalregulated kinases 1 and 2 (ERK1/2)/phosphorylated ERK1/ (pERK1/2) pathways in chronic myeloid leukemia (K562) cells. Materials and Methods: Chronic myeloid leukemia cells were cultured. Cells were incubated in the presence of muscarinic receptor agonist, antagonist and epidermal growth factor (EGF). To detect MAPK activation, ERK/pERK protein expression levels were determined by western blot method techniques. Results: Our study results showed that cholinergic agents and EGFs affect the MAPK pathway in the human K562 cell line. Conclusion: Cholinergic and EGF receptors may affect the MAPK pathway in K562 cells. Conclusion: Cholinergic and EGF receptors may affect the MAPK pathway in K562 cells