Akademik Veri Yönetim Sistemi
Applications of Chemistry in Nanosciences and Biomaterials Engineering NanoBioMat 2023 – Summer Edition, Bucuresti, Romanya, 26 - 30 Haziran 2023, ss.50-51
Bone diseases or defects cause significant problems for people around the world, both in terms of the
economy and their health. The deficiencies in traditional methods have led researchers to focus on bone
tissue engineering (BTE) and explore new and improved methods. In this study, the production and
characterization of microparticles (MPs) for BTE are emphasized. By using the polylactic acid (PLA),
bioactive glass (BG), and caffeic acid (CA) electrospray (ES) method with optimum parameters, four
different types of MPs (3% PLA, 3% PLA/0.1% BGs, 3% PLA/0.1% CA, and 3% PLA/0.1% BGs/0.1%
CA) were produced. Scanning electron microscopy (SEM) analysis revealed an increase in the mean
diameter with the addition of substances. Fourier transform infrared spectroscopy (FTIR) analysis
confirmed the presence of the main material, PLA. Differential scanning calorimetry (DSC) analysis
demonstrated that the added substances affected the thermal properties of PLA. X-ray diffraction (XRD)
analysis showed that the substances had minimal impact on the PLA pattern. In addition, in the in vitro drug
release test, the release characteristics of CA from MPs were examined, and as a result, different
compositions at different rates (180 min and 720 min) were determined. Moreover, the 3-(4,5-
dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay results showed that the MPs did not
show toxicity against human fetal osteoblastic (hFOB) 1.19 cell lines. In conclusion, this study provides
comprehensive information about the production and characterization of MPs and will be a promising study
for use in BTE studies thanks to the results obtained.