Biofabrication and 3D localization of multilayered cellular constructs using Laser Direct-Write and Mesoscopic Fluorescent Molecular Tomography

Tricomi B. J., Ozturk M. S., Intes X., Corr D. T.

2015 41st Annual Northeast Biomedical Engineering Conference, NEBEC 2015, New York, Amerika Birleşik Devletleri, 17 - 19 Nisan 2015 identifier

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Doi Numarası: 10.1109/nebec.2015.7117130
  • Basıldığı Şehir: New York
  • Basıldığı Ülke: Amerika Birleşik Devletleri
  • Anahtar Kelimeler: 3D Tissue Engineering, Laser Direct-Write, Mesoscopic Fluorescence Molecular Tomography, Microbeads
  • Marmara Üniversitesi Adresli: Hayır

Özet

© 2015 IEEE.Recent advances in tissue engineering applications demand micro-scale precision in both design and evaluation. Herein, we aim to meet these challenges through a combination of two innovative techniques: gelatin-based Laser Direct-Write (LDW) and Mesoscopic Fluorescence Molecular Tomography (MFMT). LDW was used to precisely pattern cell-loaded, alginate microbeads, in layer-by-layer fashion, to construct thick, multilayered cellular constructs. Then, MFMT was utilized to deeply assess and 3D-model cellular localization. The combination of these two techniques may provide the accuracy and precision needed for the analysis of tissue engineering in 3D.