A putative NF-Y complex interacting with ERD15 may positively regulate the expression of a peroxidase gene in response to stress in rapeseed (Brassica napus L.)

Wang J., Zhou M., Chen X., Hua J., Cui Q., Öner E. T., ...Daha Fazla

Environmental and Experimental Botany, cilt.228, 2024 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 228
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1016/j.envexpbot.2024.106015
  • Dergi Adı: Environmental and Experimental Botany
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Environment Index, Pollution Abstracts, Veterinary Science Database
  • Anahtar Kelimeler: ERD15, NF-Y, Protein-DNA interaction, Protein-protein interaction, PRX, Reactive Oxygen Species
  • Marmara Üniversitesi Adresli: Evet

Özet

Drought stress is one of the major constraints on crop productivity, including rapeseed (Brassica napus L.). Nuclear factors Y (NF-Ys) are important transcription factors involved in plant responses to drought and other stresses. However, the underlying molecular mechanisms remain unclear in rapeseed. By silencing BnaNF-YA9 in rapeseed and transforming BnaNF-YA9 into the Arabidopsis mutant Atnf-ya5, we demonstrated that BnaNF-YA9 plays a positive role in drought resistance. To explore its regulatory mechanism, we performed protein-protein interaction analyses using various approaches. Our study revealed complex interactions among BnaNF-YA9, BnaNF-YB2, BnaNF-YC4, and EARLY RESPONSIVE TO DEHYDRATION 15 (ERD15), suggesting that these proteins form a multimember complex. We also showed that BnaNF-YA9 binds to the CCAAT element in the promoter of a BnaPRX gene, which encodes a peroxidase. Interestingly, overexpression of BnaNF-YC4 or BnaERD15 in Arabidopsis increased sensitivity to salt stress, drought, and abscisic acid. Our results support an NF-Y/ERD15/PRX cascade and suggest a complex regulatory network in rapeseed that may be important in maintaining ROS homeostasis during abiotic stress responses. Our findings provide insights into potential targets for improving drought resilience in crops.