Concordance of PD-L1 expression and CD8(+) TIL intensity between NSCLC and synchronous brain metastases


Batur S., Dulger O., Durak S., Yumuk P. F., Caglar H., Bozkurtlar E., ...Daha Fazla

BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES, cilt.20, sa.3, ss.329-335, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20 Sayı: 3
  • Basım Tarihi: 2020
  • Doi Numarası: 10.17305/bjbms.2019.4474
  • Dergi Adı: BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.329-335
  • Anahtar Kelimeler: Programmed death-ligand 1, non-small-cell lung cancer, NSCLC, tumor immunology, PD-L1, brain metastasis, CD8 lymphocytes, tumor-infiltrating lymphocytes, TILs, TUMOR-INFILTRATING LYMPHOCYTES, ANTIBODY, SAFETY
  • Marmara Üniversitesi Adresli: Evet

Özet

Programmed death-ligand 1 (PD-L1) is suggested to be a predictive biomarker in non-small-cell lung carcinoma (NSCLC). However, the differential expression of PD-L1 in primary lung tumor vs. synchronous metastases, especially brain metastasis (BM), remains unclear. This study assessed the concordance of PD-L1 expression on tumor cells and tumor-infiltrating lymphocytes (TILs) and CD8(+) TIL intensity between primary lung tumors and synchronous BMs from 24 NSCLC patients. PD-L1, CD3, and CD8 positivity was determined by immunohistochemistry (IHC). PD-L1 scoring was based on the proportion of tumor cells with membranous expression of PD-L1 and the cutoff values <1%, 1-49%, >= 50%. CD3 and CD8 positivity in TILs was evaluated semi-quantitatively and the proportion of CD3+/CD8(+)TILs was determined. PD-L1 expression on tumor cells and TILs was evaluated in relation to CD3+/CD8(+)TIL proportions and the intensity of CD8(+)TILs between the paired primary lung and BM tissues. In the primary lung tumors, PD-L1 positivity was observed in 25%, 37.5%, and 37.5% cases for the cutoff values <1%, 1-49%, >= 50% respectively. PD-L1 expression on tumor cells was strongly correlated between the paired primary lung and BM tissues, in all cutoff groups. However, PD-L1 expression on TILs and the proportion of CD3+/CD8(+)TILs were not strongly correlated in all three groups between the paired primary lung tumors and BMs. The intensity of CD8(+)TILs was concordant in only 54.16% of the paired primary lung tumors and BMs. This study showed a high concordance of PD-L1 expression in neoplastic cells between primary NSCLC and synchronous BMs.