Molecular Prevalence of Giardia duodenalis and Subtype Distribution (Assemblage E and B) in Calves in Siirt, Turkey


Aslan Çelik B., Çelik Ö. Y., Ayan A., Akyıldız G., Orunç Kilinç Ö., Oktay Ayan Ö., ...Daha Fazla

EGYPTIAN JOURNAL OF VETERINARY SCIENCE, cilt.54, sa.3, ss.457-463, 2023 (ESCI) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 54 Sayı: 3
  • Basım Tarihi: 2023
  • Doi Numarası: 10.21608/ejvs.2023.192354.1440
  • Dergi Adı: EGYPTIAN JOURNAL OF VETERINARY SCIENCE
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus
  • Sayfa Sayıları: ss.457-463
  • Anahtar Kelimeler: Calves, Giardia duodenalis, Siirt, Turkey, CRYPTOSPORIDIUM SPP., DAIRY CALVES, CATTLE, IDENTIFICATION, VAN
  • Marmara Üniversitesi Adresli: Evet

Özet

Giardia duodenalis is a common intestinal protozoan parasite that infects domestic and wild mammals, birds, and humans, especially livestock, worldwide. Studies on the prevalence of Giardia duodenalis in calves in Turkey are very limited. The aim of this study was to determine the molecular prevalence and subtype distribution of Giardia duodenalis in calves in Siirt. The animal material of the study consisted of 100 calves of different breeds and sexes in Siirt province. Using a disposable latex glove, feces samples were taken from the rectum of each calf and put in separate fecal containers. Sex and age information was recorded for each sample collected. As a result, 5% and 9% positivity was detected by microscopic and nested-PCR methods, respectively. The prevalence was 9.68% in females and 7.89% in males (P>0.05). Prevalence by age groups was as follows: 11.32% in the 1-6 month group, 7.41% in the 7-12 month group, and 5% in the older than 12 months group (P>0.05). As a result of the sequence analysis of the five PCR-positive samples, 80% Assemblage E and 20% Assemblage B were detected. As a result of this study, in addition to Assemblage E, Assemblage B was detected as well, which also has zoonotic properties. This situation may pose a risk for breeders. To better understand the distribution of G. duodenalis assemblages in calves in Siirt, studies in larger herds are needed.