European Biotechnology Congress, Valencia, İspanya, 11 - 13 Nisan 2019, ss.1
Metal nanoparticles constitute a new class of antibacterial agents and several wound dressings impregnated with silver or copper nanoparticles have already been developed for the inhibition wound infections. Nanoparticles synthesized through chemical or physical routes often have limited stability and are rarely monodisperse in size. As a result studies are directed towards biological methods. Herein, the leaf extract of Ocimum sanctum, i.e. a medicinal plant renowned with antibacterial activity of its essential oil, was utilized for the green synthesis of silver nanoparticles from AgNO3 solution. Nanoparticles were characterized by UV-Vis, FT-IR, TEM and XRD techniques. Accordingly, highly crystalline nanoparticles with mean particle size of 35 nm were synthesized within 5 minutes of operation conducted under room temperature at pH 8. The zeta potential was found to be -67.6 mV, indicating strong repulsion. Thus, a stable dispersion was obtained. The nanoparticles were embedded in chitosan/polyvinyl alcohol (CS/PVA) films at various concentrations and enhanced antibacterial activity was recorded against two common pathogens, i.e Staphylococcus aureus and Pseudomonas aeruginosa.