M2 and M3 Muscarinic Receptor Expression in Bladder Cancer

Asutay K., Aydın Omay B., Filinte D., Cabadak H., Tinay İ.

ICOFMEP 2021 Congress Proceedings Book Paperback – July 9, 2021 by Bahar Uslu (Ed ) (Author), Ahmet 350en (Ed ) (Author), Kenan Demir (Ed ) (Author), Asutay K.,Aydın Omay B.,Toper M. H.,Filinte D.,Cabadak H.,Tinay İ, Editör, Scholars' Press (July 9, 2021), İstanbul, ss.161-170, 2021

  • Yayın Türü: Kitapta Bölüm / Diğer
  • Basım Tarihi: 2021
  • Yayınevi: Scholars' Press (July 9, 2021)
  • Basıldığı Şehir: İstanbul
  • Sayfa Sayıları: ss.161-170
  • Editörler: Asutay K.,Aydın Omay B.,Toper M. H.,Filinte D.,Cabadak H.,Tinay İ, Editör
  • Marmara Üniversitesi Adresli: Evet

Özet

M2 and M3 Muscarinic Receptor Expression in Bladder Cancer Cells Dr. Mehmet Kazim Asutay 1 1 Marmara University School of Medicine Department of Urology, Istanbul, Turkey E-mail 1 : kazimasutay@hotmail.com ORCID 1 : https://orcid.org/0000-0002-1822-5254 Dr. Banu Aydın 2 2 Marmara University School of Medicine Department of Biophysics, Istanbul, Turkey E-mail 2 : banu.aydin@gmail.com ORCID 2 : https://orcid.org/0000-0002-3267-8620 Dr. Hasan Toper 3 3Marmara University School of Medicine Department of Pathology, Istanbul, Turkey E-mail 3 : hasantoper@gmail.com ORCID 3 : https://orcid.org/0000-0002-2009-6164 Dr. Deniz Filinte 4 4Marmara University School of Medicine Department of Pathology, Istanbul, Turkey E-mail 4 : filinte@yahoo.com ORCID 4 : https://orcid.org/0000-0002-8980-7665 Dr. Hülya Cabadak 5 5Marmara University School of Medicine Department of Biophysics, Istanbul, Turkey E-mail 5 : hcabadak@gmail.com ORCID 5 : https://orcid.org/0000-0002-5757-2198 Dr. İlker Tinay 6 6Marmara University School of Medicine Department of Urology, Istanbul, Turkey E-mail 6 : ilker_tinay@yahoo.com ORCID 6 : https://orcid.org/0000-0002-6768-9373 Abstract Diagnosis of bladder cancer represents a heterogeneous group of cancers, where urothelial cell carcinoma constitutes 90% of all histological subtypes. Muscarinic receptors, M2 and M3 type receptors are important for the function of the bladder tissue and common targets for treatment. In this study, muscarinic receptor expression, namely M2 and M3 subtypes of muscarinic receptors were studied in bladder tumour samples. Bladder, cancer tissue samples were collected during transurethral resection and were preserved at -80°C. Bladder tumour tissues were analysed by Western blot (WB) technique, and immune-histochemistry (IHC). Anti-M2 and anti-M3 antibodies were performed in bladder cancer tissue samples. Relationship between M2 and M3 receptor density and with patient age, gender, pathological stage, histological grade, presence of smoking, EORTC (European Organization for Research and Treatment of Cancer) risk group, presence of progression, history of intracavitary BCG and presence of recurrence were analysed. The data of 45 patients with different pathological T stages and tumour grades were analysed. pT2 tumours exhibited higher amounts of M2 receptors and M2 receptor percentages were also significantly higher in cases that showed progression during follow-up on IHC workup. There was no relation between M3 muscarinic receptor level and tumour grade/stage or progression rate. M2 and M3 muscarinic receptors may be important for bladder cancer patho-physiology though their role in the development of bladder cancer has not been defined yet. This study with limited number of patients shows a potential for M2 receptors as a target for bladder cancer treatment. Keywords: Bladder cancer, muscarinic receptor, molecular oncology, urothelial carcinoma