Preparation of Core-Shell Type Polyacrylate/Hydroxyapatite Drug Carriers by Controlled Polymerization


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Kayaman Apohan N. , Koçal G., Oktay B.

uluslararası 6. ilaç kimyası kongresi, Antalya, Türkiye, 22 - 25 Mart 2018, ss.54

  • Basıldığı Şehir: Antalya
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.54

Özet

Preparation of core-shell type polyacrylate/hydroxyapatite drug carriers by controlled polymerization

 

Gamze Koçal, Burcu Oktay and Nilhan Kayaman Apohan

 

Marmara University, Chemistry Department, Faculty of Arts and Sciences, Göztepe, Istanbul

 

E-mail: gamzekocall@gmail.com

 

 

Synthetic hydroxyapatite (HA) is a material commonly used in biomaterial engineering and regenerative medicine for bones [1,2]. Actually, the natural bone composes of an organic component such as collagen and an inorganic component such as HA. The inorganic apatite compound offers osteoconductivity, osteoinductivity and a bone-bonding ability in the process of bone growth or bone healing [3,4]. Biological apatite is a nanocrystalline multi-substituted carbonated HA, which deficient in calcium and with a reduced number of structural hydroxyl groups. This characteristic composition provides apatite with unique physicochemical and biological properties.  HA is particularly useful as a drug carrier since it has biocompatible, non-toxic and inert in the corrosive body fluid environment. The porous hydroxyapatite exhibits good physical-chemical properties such as large surface area, uniform pore size, high pore volume, which allow multiple drug loading, slow and controlled release.

The goal of this study is to prepare HA based controlled drug delivery systems with high biocompatibility using the atom transfer radical polymerization (ATRP). For this reason, we synthesized meso–porous hydroxyapatite (MHA) by using hydrothermal method in the presence of cetyltrimethylammonium bromide (CTAB). Since the -OH groups on the surface of the meso-porous hydroxyapatite are less active and their surface has been modified with NH2. For this purpose, we used aminopropyltriethoxysilane (APTES). ATPES shows good binding properties to both organic and inorganic molecules for modification. To obtain HA-based ATRP initiator (MHA-Br), the NH2 groups of the MHA were then brominated with 2-bromoisobutyryl bromide. Afterwards, ATRP of amino methacrylamide (DMAM), fluorescein methacrylate (FMA) and polyethylene glycol monomethacrylate (PEGMA) monomers were performed on the MHA-Br.  The prepared novel materials were characterized via FT-IR, TGA and DSC. The morphology of the drug carrier was investigated by STEM and ESEM.

 

 

References

 

[1] S.V. Dorozhkin, Calcium orthophosphate-based bioceramics, Materials 6 (9) (2013) 3840–3942.

[2] S. Bose, S. Tarafder, A. Bandyopadhayay, Hydroxyapatite coatings formetallic implants, in: M. Mucalo (Ed.), Hydroxyapatite (HAp) for Biomedical Applications, Woodhead Publishing series in Biomaterials,Cambridge, UK, 2015, pp. 143–159.

[3] Chen F, Tang QL, Zhu YJ, Wang KW, Zhang ML, Zhai WY, Chang J. Hydroxyapatite

nanorods/pol(yvinyl pyrolidone) composite nanofibers, arrays and three-dimensional fabrics: electrospun preparation and transformation to hydroxyapatite nanostructures.Acta Biomaterialia, 2010, 6, 3013-3020.

[4] Bang LT, Othman R. Aging time and synthesis parameters of nanocrystalline single phase hydroxyapatite produced by aprecipitation method. Ceramics –Silikáty, 2014, 58, 157-164

 

 

 Preparation of core-shell type polyacrylate/hydroxyapatite drug carriers by controlled polymerization

 

Gamze Koçal, Burcu Oktay and Nilhan Kayaman Apohan

 

Marmara University, Chemistry Department, Faculty of Arts and Sciences, Göztepe, Istanbul

 

E-mail: gamzekocall@gmail.com

 

 

Synthetic hydroxyapatite (HA) is a material commonly used in biomaterial engineering and regenerative medicine for bones [1,2]. Actually, the natural bone composes of an organic component such as collagen and an inorganic component such as HA. The inorganic apatite compound offers osteoconductivity, osteoinductivity and a bone-bonding ability in the process of bone growth or bone healing [3,4]. Biological apatite is a nanocrystalline multi-substituted carbonated HA, which deficient in calcium and with a reduced number of structural hydroxyl groups. This characteristic composition provides apatite with unique physicochemical and biological properties.  HA is particularly useful as a drug carrier since it has biocompatible, non-toxic and inert in the corrosive body fluid environment. The porous hydroxyapatite exhibits good physical-chemical properties such as large surface area, uniform pore size, high pore volume, which allow multiple drug loading, slow and controlled release.

The goal of this study is to prepare HA based controlled drug delivery systems with high biocompatibility using the atom transfer radical polymerization (ATRP). For this reason, we synthesized meso–porous hydroxyapatite (MHA) by using hydrothermal method in the presence of cetyltrimethylammonium bromide (CTAB). Since the -OH groups on the surface of the meso-porous hydroxyapatite are less active and their surface has been modified with NH2. For this purpose, we used aminopropyltriethoxysilane (APTES). ATPES shows good binding properties to both organic and inorganic molecules for modification. To obtain HA-based ATRP initiator (MHA-Br), the NH2 groups of the MHA were then brominated with 2-bromoisobutyryl bromide. Afterwards, ATRP of amino methacrylamide (DMAM), fluorescein methacrylate (FMA) and polyethylene glycol monomethacrylate (PEGMA) monomers were performed on the MHA-Br.  The prepared novel materials were characterized via FT-IR, TGA and DSC. The morphology of the drug carrier was investigated by STEM and ESEM.

 

 

References

 

[1] S.V. Dorozhkin, Calcium orthophosphate-based bioceramics, Materials 6 (9) (2013) 3840–3942.

[2] S. Bose, S. Tarafder, A. Bandyopadhayay, Hydroxyapatite coatings formetallic implants, in: M. Mucalo (Ed.), Hydroxyapatite (HAp) for Biomedical Applications, Woodhead Publishing series in Biomaterials,Cambridge, UK, 2015, pp. 143–159.

[3] Chen F, Tang QL, Zhu YJ, Wang KW, Zhang ML, Zhai WY, Chang J. Hydroxyapatite

nanorods/pol(yvinyl pyrolidone) composite nanofibers, arrays and three-dimensional fabrics: electrospun preparation and transformation to hydroxyapatite nanostructures.Acta Biomaterialia, 2010, 6, 3013-3020.

[4] Bang LT, Othman R. Aging time and synthesis parameters of nanocrystalline single phase hydroxyapatite produced by aprecipitation method. Ceramics –Silikáty, 2014, 58, 157-164