Akademik Veri Yönetim Sistemi
3rd INTERNATIONAL EURASIAN MYCOLOGY CONGRESS (EMC’22), Van, Türkiye, 7 - 09 Eylül 2022, ss.112-113, (Özet Bildiri)
Lepista nuda (Bull. ex Fr). Cooke (Tricholomataceae) is utilized in public medicine. The sporophore of L. nuda includes Vit B. Infusion1 of this macrofungus is utilized to prevent beriberi. Moreover, decoctions are utilized for the therapy of abscesses and wounds. It possesses significant nutritional and medicinal importance, and particularly has high antioxidant, antitumor and antiviral effects. It is an edible fungus and its cap is lavender, and its round size is (6.1–8) × (4.0–5) μm. This is an unusual, wild mushroom, which includes perfect food constituents. Its cap includes numerous nutrients, containing 56.39% protein, 45.0% total sugar, 3.87% fat, and 7.92% fiber, with anti-vascular sclerosis, anti-virus, anti-gallstone, and anti-diabetes effects. It has been reported that regulates glucose metabolism. Mushrooms have been known as a significant component of gourmet cuisine global with their perfect flavors, nutritious properties and aromas. Owing to their significant nutritional properties, fungi, that are approved as nutraceutical foods are enhancing their interest owing to their medicinal properties and economic significance. In this study, in vitro antidiabetic, antioxidant, antimicrobial activities and anatomical properties of L. nuda have been performed. The mushroom (50 g) was powdered and macerated with methanol at room temperature for 8 h/3 days with a mechanical mixer. The methanolic extract evaporated to dryness and was dispersed in a mixture of methanol: water (1:9), then fractionated with solvents of different polarities (n-hexane, dichloromethane, ethyl acetate, n-butanol), respectively, and the sub-extracts gained via evaporating with a rotatory evaporator were weighed. Detection of α-Amylase Enzyme Inhibition Detection of α-amylase enzyme inhibition effect Nampoothiri et al. (2011). Acarbose was used as a positive control. Detection of α-Glucosidase Enzyme Inhibition α-glucosidase enzyme inhibition test, Bachhawat et al. (2011) method was applied by making some modifications. Acarbose was used as a positive control. Detection of Total Phenolic Component The total phenolic compound amounts of the extracts gained from the mushroom were determined utilizing the method developed by Folin and Denis and modified by Singleton. Gallic acid was used as the standard phenolic compound. DPPH• radical-scavenging activity the DPPH• radical scavenging activities of extracts obtained from the mushroom were tested according to the Blois method. 1 mM DPPH• solution was used as a free radical, and α-tocopherol and trolox were used as standard antioxidants. ABTS•+ radical-scavenging activity ABTS•+ cation radical scavenging activities were determined according to the study by Re (Re et al. 1999). 2 mM ABTS•+ solution was used as free radical, and α-tocopherol and trolox were used as standard antioxidants. Antimicrobial Activity Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6538, Candida albicans ATCC 10231 antimicrobial activity was performed using microorganism strains. Extracts were prepared at a concentration of 1 mg/ml. Moxifloxacin, ampicillin, and fluconazole used as standard were prepared at 128 µg/ml microscopic analysis. The investigations were made in a Jeol JSM 6490LV scanning electron microscope at the Turkish Petroleum International Company (TPAO) Research Centre SEM laboratory, Ankara. Results and Discussion: Determination of α-Amylase Enzyme Inhibition The hexane extract showed the best α-amylase inhibition activity at 5000 µg/ml with 11.22% (38.65% for acarbose). It showed lower activity than acarbose. Determination of α-Glucosidase Enzyme Inhibition The hexane extract showed the best αglucosidase inhibition activity at 5000 µg/ml with 10.26% (56.59% for acarbose). It showed lower activity than acarbose. Determination of Total Phenolic Component Butanol and Aquas and Ethyl Acetate extracts showed the best total phenolic component with 4.375 µg GAE/ mg, 0.125 µg GAE/ mg ,4.312 µg GAE/ mg extracts. Other extracts were found to be lower. DPPH• radical-scavenging activity Butanol extract showed the best DPPH• radical scavenging activity with 9.221% at 100 µg/ml. It was found to be very low compared to the standards (α-tocopherol 91.957%, trolox 93.317%). ABTS•+ radical-scavenging activity Butanol with 25.111% showed the best ABTS•+ radical scavenging activitiy at 60 µg/ml. It was found to be lower than the standards (α-tocopherol 90.127%, trolox 96.597%). Antimicrobial Activity Ethyl acetate extract showed the best antimicrobial activity against E. coli ATCC 8739, S. aureus ATCC 6538, and C. albicans ATCC 10231 with 250, 250, and 125 µg/ml MIC values, respectively. Microscopic analysis According to anatomy studies, the shape of spore of mushroom was found as spheroidal and size of spore was determined as 4.9-5.5 μm. Acknowledgment: BİLGE Aydın and Enes TEKMAN would like to acknowledge the scholarship during their postgraduate program provided by the Turkish Scientific and Technical Research Council (TUBITAK).
KEYWORDS: Lepista nuda, antioxidant, antimicrobial, antidiabetic, total phenolic, spore, microscopic