Methylnitrosourea, dimethylbenzanthracene and benzoapyrene differentially affect redox pathways, apoptosis and immunity in zebrafish

Eğimezer G., Üstündağ Ü., Ateş P., Ünal I., Üstündağ F., Alturfan A., ...Daha Fazla

HUMAN & EXPERIMENTAL TOXICOLOGY, cilt.39, sa.7, ss.920-929, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39 Sayı: 7
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1177/0960327120905961
  • Dergi Adı: HUMAN & EXPERIMENTAL TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, Chimica, CINAHL, EMBASE, Environment Index, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.920-929
  • Anahtar Kelimeler: Methylnitrosourea, dimethylbenzanthracene, benzoapyrene, antioxidant, apoptosis, immunity, zebrafish, NITRIC-OXIDE SYNTHASE, METHYL-N-NITROSOUREA, OXIDATIVE STRESS, SUPEROXIDE-DISMUTASE, INTERFERON-GAMMA, IN-VIVO, CANCER, DMBA, EXPRESSION, EXPOSURE
  • Marmara Üniversitesi Adresli: Evet

Özet

Cancer continues to be a major cause of mortality globally. Zebrafish present suitable models for studying the mechanisms of genotoxic carcinogens. The aim of this study was to investigate the interaction between oxidant-antioxidant status, apoptosis and immunity in zebrafish that were exposed to three different genotoxic carcinogens methylnitrosourea, dimethylbenzanthracene, benzoapyrene and methylnitrosourea + dimethylbenzanthracene starting from early embryogenesis for 30 days. Lipid peroxidation, nitric oxide levels, superoxide dismutase and glutathione-S-transferase activities and mRNA levels of apoptosis genes p53, bax, casp3a, casp2 and immunity genes fas, tnf alpha and ifn gamma 1 were evaluated. The disruption of the oxidant-antioxidant balance accompanied by altered expressions of apoptotic and immunity related genes were observed in different levels according to the carcinogen applied. Noteworthy, ifn gamma expressions decreased in all carcinogen-exposed groups. Our results will provide basic data for further carcinogenesis research in zebrafish models.