Refining measurement techniques for N2H4, NH2OH, and NH4+-N in nitrogen-rich environments: Mitigating interference for accurate detection

Sari T., AKGÜL D.

Process Biochemistry, cilt.156, ss.317-328, 2025 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 156
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1016/j.procbio.2025.06.008
  • Dergi Adı: Process Biochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Compendex, Food Science & Technology Abstracts, INSPEC, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.317-328
  • Anahtar Kelimeler: Ammonia, Anammox, Hydrazine, Hydroxylamine, Interference, Nitrite, Pretreatment
  • Marmara Üniversitesi Adresli: Evet

Özet

In biological studies, several nitrogenous compounds interfere with each other's measurements, which may result in unreliable results and misinterpretation. In this context, this study aims to refine the analytical techniques for N2H4, NH2OH, and NH4+-N by eliminating interferences to obtain accurate data from the anammox system as a case study. Results indicated that NO2--N interferes with N2H4 and NH2OH measurements, while N2H4 and NH2OH interfere with NH4+-N measurements. In N2H4 determination, different NO2--N levels resulted in different utilization of sulfamic acid (SA) in terms of volume and concentration. The samples containing ≤ 2 mg/L NO2--N were pretreated with 400 µL 0.5 % SA to prevent NO2--N interference, while 10 % SA is required for NO2--N concentrations between 27.25 mg/L and 46.62 mg/L. The 10 % SA treatment also significantly mitigated NH2OH degradation in solutions, ensuring precise and steady results even in the presence of NO2--N. Adding 0.1 g of KIO3 to 50 mL of samples containing N2H4 (≤ 10 mg/L) produced findings most comparable to the control group in NH4+-N tests. Furthermore, without any pretreatment, it is possible to measure NH4+-N accurately if the NH2OH concentration is ≤ 0.01 mM. Overall, this study offers insightful data for further biological research in nitrogen-rich environments.