Comparison of MALDI-TOF MS, nucleic acid hybridization and the MPT64 immunochromatographic test for the identification of M-tuberculosis and non-tuberculosis Mycobacterium species


Samli A., Ilki A.

NEW MICROBIOLOGICA, vol.39, pp.259-263, 2016 (Journal Indexed in SCI) identifier

  • Publication Type: Article / Article
  • Volume: 39
  • Publication Date: 2016
  • Title of Journal : NEW MICROBIOLOGICA
  • Page Numbers: pp.259-263
  • Keywords: Mycobacteria, MALDI-TOF MS, MPT ICT, Nucleic acid hybridization, DESORPTION IONIZATION-TIME, MASS-SPECTROMETRY, RAPID IDENTIFICATION, CULTURE-MEDIA, DIRECT ASSAY, COMPLEX

Abstract

Mycobacteria are an important cause of morbidity in humans. Rapid and accurate mycobacterial identification is important for improving patient outcomes. However, identification of Mycobacterium species is not easy, due to the slow and fastidious growth of mycobacteria. Recently, biochemical, sequencing, and probing methods have come to be used for identification. This study compared the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification of M. tuberculosis and non-tuberculosis Mycobacteria (NTM) to those of nucleic acid hybridization (NAH) and the MPT64 immunochromatographic test.